bacterial cell wall homeostasis methods and protocols methods in molecular biology book 1440

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Bacterial Cell Wall Homeostasis

Author : Hee-Jeon Hong
ISBN : 1493936743
Genre : Science
File Size : 61. 51 MB
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This volume brings together the most widely used and important protocols currently being employed in researching and understanding bacterial cell wall homeostasis. Chapters in Bacterial Cell Wall Homeostasis cover a variety of subjects, such as: modern microscopy techniques and other biophysical methods used to characterize the subcellular structure of the bacterial cell wall; high-throughput approaches that can be used to identify all the genes and proteins that participate in the correct functioning of an organism’s cell wall; protocols for assaying individual gene products for specific cell wall functions or identify chemicals with inhibitory activity against the cell wall; and methods for analyzing the non-protein components of the cell wall and the increasing use of computational approaches for predicting and modeling cell wall related functions and processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocols emphasizes the diversity of the research taking place in bacterial cell wall homeostasis, and explains how the integration of information from across multiple disciplines is going to be essential if a holistic understanding of this important process is to be obtained.

Molecular Biomethods Handbook

Author : John M. Walker
ISBN : 1603273743
Genre : Science
File Size : 88. 76 MB
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Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.

Metabolome Analysis

Author : Silas G. Villas-Boas
ISBN : 9780470105504
Genre : Science
File Size : 81. 26 MB
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Providing information on the main approaches for the analysis of metabolites, this textbook: Covers basic methodologies in sample preparation and separation techniques, as well as the most recent techniques of mass spectrometry. Differentiates between primary and secondary metabolites. Includes four chapters discussing successful metabolome studies of different organisms. Highlights the analytical challenges of studying metabolites. Illustrates applications of metabolome analysis through the use of case studies.

The Science Of Flavonoids

Author : Erich Grotewold
ISBN : 9780387288222
Genre : Science
File Size : 52. 31 MB
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This is the only book of its kind to provide an overview of the science of flavonoids in plants.

Bioactive Compounds From Natural Sources

Author : Corrado Tringali
ISBN : 0203212312
Genre : Science
File Size : 74. 90 MB
Format : PDF
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Interest in obtaining biologically active compounds from natural sources has recently spiked due to their low toxicity, complete biodegradability, availability from renewable sources, and in most cases, low cost. Taking an interdisciplinary approach, Bioactive Compounds from Natural Sources: Isolation, Characterization, and Biological Properties covers general methods and main topics in the research field of bioactive natural products. The book describes general screening methods, modern HPLC hyphenated techniques, and NMR methods in the structural elucidation of compounds and devotes individual chapters to specific topics of research. Surveys on compounds displaying important pharmacological activities are presented in chapters devoted to Mexican medicinal plants, anti-tumor drugs of natural origin, cancer chemopreventive flavonoids, and metabolites displaying anti-HIV, antioxidative, antimalarial, and anti-inflammatory activity. The final chapters are devoted to representative examples of research into marine metabolites: immunomodulating marine glycolipids and surveys of bioactive compounds from marine opisthobranchs and Japanese soft corals. With its focus on modern approaches to the isolation of biologically active natural products, this book encourages interdisciplinary work among chemists, pharmacologists, biologists, botanists, and agronomists with an interest in bioactive natural products.

Molecular Biology

Author : Susan J. Karcher
ISBN : 0080536832
Genre : Science
File Size : 38. 82 MB
Format : PDF, ePub
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This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Key Features * Includes a glossary of over 200 terms important for understanding molecular biology * Uses an inexpensive source of eukaryotic cells - great for schools on a budget * Includes Methods Locator that provides instant access to the latest methods * Contain clearly written, easy-to-follow, student-tested instructions: * Sterile techniques * Phage titration * Gel electrophoresis of DNA * Restriction enzyme digestion * Plasmid isolation * Transformation of E. Coli * Recombinant DNA cloning * Nick translation labeling * Nonradioactive primer labelling * Nonradioactive DNA detection * Southern blotting * Colony hybridization * Purification of plant DNA * RNA purification * Northern blotting * Purification of poly A+ RNA * Polymerase chain reaction (PCR)

Alone On The Wall

Author : Alex Honnold
ISBN : 0393353176
Genre :
File Size : 59. 68 MB
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The life and death-defying feats of Alex Honnold, a visionary climber of the sort that comes along only once in a generation.

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